2/10/2024 0 Comments Jing gao geisel medical schoolCollectively, our data shed new light on the roles of and mechanisms involved in the repression of DCs by miRNAs, which may contribute to efforts to develop a prophylaxis for the influenza virus.įrequent outbreaks of reassortant H7N9 avian influenza virus (AIV) caused great threatened to the poultry industry and the public health. Among them, Mbnl3 interference strongly blocked the mature DCs. Furthermore, we predicted and demonstrated that Pgm2l1, Aldh18a1, Camk1d, and Mbnl3 were the target genes of miR-674. Nevertheless, by inhibiting the expression of miR-674 and miR-155, NA lost the ability to promote DC maturation. Meanwhile, we found that miR-674 and miR-155 over-expression increased all surface markers of DC. Our results suggest that NA significantly increased DC surface markers CD80 and MHCII and enhanced the ability of activating lymphocytes and secreting cytokines compared with HA, NP and M2. Then, we showed that, consistent with the H9N2 virus treatment, the viral NA up-regulated the expression of miR-155, miR-674, and miR-499 in DCs however, unlike H9N2 virus treatment, the presence of NA was associated with reduced expression of miR-181b1. First, we screened for and verified the miRNAs induced by NA. Here, we studied the molecular mechanism of miRNA-induced repression of immune responses in mouse DCs. Dendritic cells (DCs) are major antigen-presenting cells whose immune functions, such as presenting antigens and activating lymphocytes, can be regulated by microRNAs. Neuraminidase (NA), a structural protein of the H9N2 avian influenza virus (H9N2 AIV), can facilitate viral invasion of the upper airway by cleaving the sialic acid moieties on mucin. Received: NovemAccepted: Published: JAbstract Keywords: dendritic cells neuraminidase miR-674 immune regulation virus replication Immunology and Microbiology Section Immune response Immunity Chen 1, Jing Xia 1 and Qian Yang 2ġ Life Science College, Nanjing Agricultural University, Weigang, Nanjing, Jiangsu, PR ChinaĢ College of Veterinary Medicine, Nanjing Agricultural University, Weigang, Jiangsu, PR China
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